| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Product Type | |
| Signaling Pathway | |
| Target |
Compound Overview
F-Amidine TFA (CAS 877617-46-4) is a research-grade small molecule supplied by TargetMol.
Physical Properties
| CAS Number | 877617-46-4 |
|---|---|
| Form | Solid |
| Shipping | Room Temperature (RT) |
Biological Activity
F-amidine is a selective inhibitor of protein arginine deiminases (PADs), specifically targeting PAD1 and PAD4 with in vitro IC50 values of 29.5, 350, and 21.6 uM for PAD1, PAD3, and PAD4, respectively. It irreversibly inactivates all four PAD subtypes by covalently modifying an active site cysteine crucial for enzymatic activity, with kinact/KI values of 2,800, 380, 170, and 3,000 M^-1min^-1. Additionally, F-amidine demonstrates cytotoxicity against HL-60, MCF-7, and HT-29 cancer cell lines, with IC50s of 0.5, 0.5, and 1 uM, respectively.
Safety & Regulatory
For Research Use Only (RUO). Not for therapeutic, diagnostic, or clinical use. Wear appropriate PPE and consult the Safety Data Sheet (SDS) prior to handling.
Due to the small pack, the powder may be highly dispersed during shipping due to static electricity and may adhere to the vial. Please centrifuge and add an appropriate solvent to fully dissolve the product and prepare the stock solution.
Product quality is assured — all TargetMol compounds undergo structural confirmation by ¹H NMR and purity determination by HPLC before release. If no effect is observed, consider the following: (1) the IC50 in literature may have been determined in a different cell line or assay format; (2) solubility or cell permeability may be limiting at the tested concentration; (3) establish a concentration gradient (e.g., 0.1–30 µM) tailored to your specific experimental system. A positive control compound with a known cellular effect in your assay is recommended.
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
To quote quickly, tell us: compound name + CAS/structure (SMILES or mol file), intended assay context, solvent preference, salt/stereochemistry requirements, purity/QC level, and the amount (mg–g).
Can’t find the compound you’re looking for?
Send the CAS or structure and your specs. We can help source it, suggest close
equivalents, or discuss custom synthesis with the right QC documentation (RUO).
