| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human FBXO7 recombinant protein (Position: E18-M522) was used as the immunogen for the FBXO7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
FBXO7 Antibody / F-box only protein 7 is a anti-FBXO7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: FBXO7
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IP, FACS, ELISA
Biological background
Functionally, FBXO7 antibody identifies a 522-amino-acid cytoplasmic and nuclear protein containing an F-box domain for SCF complex binding and a proline-rich region that mediates protein-protein interactions. FBXO7 participates in cell cycle regulation by targeting cell cycle inhibitors for ubiquitination. It also plays a role in mitophagy by interacting with PINK1 and Parkin to promote the clearance of damaged mitochondria, maintaining neuronal and muscular health.
The FBXO7 gene is located on chromosome 22q12.3 and is broadly expressed, with high levels in brain, skeletal muscle, and lymphoid tissues. Its cellular distribution reflects multifunctional roles in ubiquitin-mediated proteolysis, cytoskeletal organization, and signal transduction.
Pathologically, mutations in FBXO7 are associated with Parkinsonian-pyramidal syndrome (PARK15), a neurodegenerative disorder combining parkinsonism with upper motor neuron dysfunction. Loss of FBXO7 disrupts mitophagy, leading to mitochondrial dysfunction and neurodegeneration. Research using FBXO7 antibody supports studies in protein degradation, neurobiology, and mitochondrial homeostasis.
FBXO7 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect F-box proteins and ubiquitination regulators.
Structurally, F-box only protein 7 contains an F-box domain for SKP1 binding, coiled-coil regions for dimerization, and a ubiquitin-like domain that facilitates substrate recruitment. This antibody enables detailed characterization of FBXO7's function in E3 ligase activity and mitochondrial quality control.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
