| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human FGD5 recombinant protein (Position: Q705-L1462) was used as the immunogen for the FGD5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
FGD5 Antibody / FYVE, RhoGEF and PH domain-containing protein 5 is a anti-FGD5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: FGD5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
FGD5 contains multiple conserved domains, including a FYVE domain that binds phosphatidylinositol 3-phosphate, a Dbl homology (DH) domain that catalyzes GTP loading of CDC42, and a pleckstrin homology (PH) domain that anchors the protein to membranes. These domains coordinate spatial activation of CDC42 at specific cellular sites, promoting actin cytoskeletal rearrangements necessary for cell migration and tube formation. The FGD5 antibody provides a key reagent for examining how this protein localizes to endosomes and plasma membrane regions during angiogenic signaling.
Endothelial FGD5 regulates cell polarity, barrier integrity, and lumen formation by activating CDC42 and downstream effectors such as PAK and N-WASP. Its expression is induced by vascular growth factors, including VEGF and FGF, which drive endothelial morphogenesis. The FGD5 antibody supports studies into these processes, helping delineate how Rho GTPase signaling orchestrates angiogenic sprouting and vessel stabilization. Loss of FGD5 function leads to impaired vascular network formation, highlighting its essential developmental role.
Beyond angiogenesis, FYVE, RhoGEF, and PH domain-containing protein 5 contributes to intracellular trafficking and signal transduction in other tissues, including kidney and brain. Abnormal FGD5 expression has been linked to vascular pathologies, diabetic retinopathy, and cancer progression, where altered endothelial behavior supports tumor angiogenesis. The FGD5 antibody allows researchers to quantify expression changes under pathological conditions and explore its regulatory influence on endothelial plasticity.
The FGD5 antibody performs well in western blotting, immunofluorescence, and immunohistochemistry, yielding distinct cytoplasmic and membrane-associated staining.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
