| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human FOXC2 recombinant protein (Position: L200-Y501) was used as the immunogen for the FOXC2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
FOXC2 Antibody / Forkhead box protein C2 is a anti-FOXC2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: FOXC2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
FOXC2 is encoded by the FOXC2 gene located on human chromosome 16q24.1. It binds to promoter regions of target genes containing the forkhead consensus sequence and activates transcription of genes involved in cell migration and morphogenesis. In the vascular system, FOXC2 controls differentiation of lymphatic endothelial cells and formation of lymphatic valves. Mutations in FOXC2 cause lymphedema-distichiasis syndrome, characterized by lymphatic dysfunction and abnormal eyelash growth.
The FOXC2 antibody is frequently used in developmental and cancer research to detect protein expression in tissue sections and cultured cells. Western blot analysis typically reveals a 50�55 kDa nuclear protein, while immunohistochemistry demonstrates nuclear staining in endothelial and mesenchymal cells. In cancer biology, FOXC2 serves as a key regulator of EMT and is upregulated in aggressive tumors, where it promotes invasion, metastasis, and resistance to chemotherapy.
FOXC2 also contributes to adipose tissue differentiation and energy homeostasis by regulating genes that determine brown adipocyte lineage. It functions in cooperation with transcription factors such as PPARgamma and PRDM16. Beyond developmental and metabolic functions, FOXC2 is implicated in cardiac valve formation and osteogenesis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
