| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A portion of amino acids 1-30 from the human protein was used as the immunogen for the FOXD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
FOXD1 Antibody / Forkhead box protein D1 is an antibody targeting FOXD1, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: FOXD1 (reported localization: Nuclear).
- Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Purified.
- Species reactivity: Human, Mouse.
- Listed applications: WB, IHC-P, IF (refer to on-page specifications for application-specific guidance).
Biological background
FOXD1 belongs to the forkhead box protein family, specifically the forkhead box D (FOX D) subfamily. It is involved in regulating gene expression by binding to specific DNA sequences and influencing transcription. FOXD1 is known to play essential roles in embryonic development, particularly in the formation of various tissues and organs. In addition, it has been implicated in the regulation of cell proliferation, differentiation, and survival. It is also involved in the development of the kidney and nervous system. Studies have shown that FOXD1 is essential for the formation of the metanephric mesenchyme, a precursor structure for the kidney. Moreover, FOXD1 has been linked to neurogenesis and neuronal differentiation, highlighting its crucial role in brain development. Dysregulation of FOXD1 expression has been associated with several diseases and disorders. For instance, aberrant expression of FOXD1 has been observed in various cancers, including breast cancer, lung cancer, and renal cell carcinoma. In these contexts, FOXD1 may act as an oncogene or tumor suppressor, depending on the cellular context and signaling pathways involved. Furthermore, FOXD1 has been implicated in the pathogenesis of neurodevelopmental disorders, such as autism and schizophrenia. Studies have suggested that dysregulated FOXD1 expression may contribute to the atypical brain development seen in these conditions.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Immunofluorescence: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
