| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human FOXM1 recombinant protein (Position: Q457-P760) was used as the immunogen for the FOXM1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
FOXM1 Antibody / Forkhead box protein M1 is a anti-FOXM1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: FOXM1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA, IP
Biological background
FOXM1 localizes to the nucleus during interphase and associates with condensed chromosomes during mitosis. Its activity is controlled by phosphorylation through CDKs and PLK1, which modulate DNA-binding and transcriptional activation. FOXM1 also contributes to chromatin remodeling and genomic stability by facilitating DNA repair and replication stress responses. It serves as an essential factor in tissue regeneration, stem cell renewal, and oncogenic transformation.
The FOXM1 antibody is widely used in cancer, developmental, and cell cycle research to investigate proliferation control and transcriptional regulation. Western blot analysis detects a 90 kilodalton band corresponding to FOXM1, while immunofluorescence shows strong nuclear staining in proliferating cells. This antibody enables assessment of mitotic activity and transcriptional control mechanisms in both normal and cancerous tissues.
Overexpression of FOXM1 is a hallmark of many human cancers, where it promotes tumor growth, angiogenesis, and metastasis through activation of cell cycle and invasion-related genes. Its inhibition is being explored as a therapeutic strategy for targeting rapidly dividing cells. The FOXM1 antibody provides a reliable tool for studying transcriptional regulation of proliferation and tumorigenesis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
