| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human FPGS was used as the immunogen for the FPGS antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
FPGS Antibody / Folylpolyglutamate synthase is a anti-FPGS Rabbit antibody Recombinant Rabbit Monoclonal clone 29F90 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: FPGS
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29F90, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Folylpolyglutamate synthase is localized to both mitochondria and cytosol, ensuring polyglutamation occurs in multiple compartments. This enzyme plays an indispensable role in maintaining pools of tetrahydrofolate derivatives required for DNA synthesis and repair. Research using FPGS antibody has revealed that reduced enzyme activity decreases intracellular folate retention and disrupts nucleotide biosynthesis, leading to impaired proliferation. These findings explain why mutations or dysregulation of FPGS contribute to megaloblastic anemia and developmental defects.
In oncology, FPGS is highly relevant because it modulates the activity of antifolate chemotherapeutics such as methotrexate and pemetrexed. These drugs require polyglutamation by FPGS for retention and enhanced potency. Research using FPGS antibody has demonstrated that low expression of Folylpolyglutamate synthase confers resistance to antifolate therapy, while high activity sensitizes cells to treatment. This makes FPGS an important biomarker for predicting drug response in leukemia, lymphoma, and solid tumors. Studies continue to evaluate whether modulation of FPGS expression can overcome resistance and improve outcomes in antifolate-based regimens.
Beyond cancer, folate metabolism plays critical roles in pregnancy, neural tube development, and cardiovascular health. Research with FPGS antibody has shown that adequate polyglutamate production is required for normal development, and deficiencies in this pathway contribute to congenital malformations and disease. As such, FPGS remains an important protein in both clinical and nutritional studies. Immunohistochemistry and western blotting using FPGS antibody have confirmed its widespread expression across tissues, with particular enrichment in proliferative and metabolically active cells.
FPGS antibody is used in western blotting, immunohistochemistry, and enzyme activity assays. Western blotting measures relative abundance in normal and tumor tissue, immunohistochemistry demonstrates tissue distribution, and enzymatic assays link FPGS expression with catalytic activity. Together, these methods provide a full picture of how Folylpolyglutamate synthase supports cell metabolism, proliferation, and therapeutic responses.
By providing validated FPGS antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
