| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human FRG1 was used as the immunogen for the FRG1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
FRG1 Antibody / FSHD region gene 1 is a anti-FRG1 Rabbit antibody Recombinant Rabbit Monoclonal clone 32F07 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: FRG1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32F07, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF
Biological background
FSHD region gene 1 protein is expressed in both nuclear and cytoplasmic compartments. In the nucleus, it associates with splicing machinery and contributes to RNA maturation. Research using FRG1 antibody has demonstrated that overexpression of FRG1 alters splicing of muscle-specific transcripts, contributing to muscle pathology. In the cytoplasm, FRG1 interacts with actin filaments, influencing cytoskeletal organization and cell motility. These dual roles highlight its broad impact on cellular function.
Overexpression of FRG1 has been shown to produce muscular dystrophy-like phenotypes in model organisms. Studies with FRG1 antibody have confirmed that excessive expression disrupts muscle development, leading to muscle weakness and degeneration. This evidence supports the hypothesis that misregulation of FRG1 contributes to FSHD pathology. Conversely, controlled levels of FRG1 appear necessary for normal muscle and vascular development, linking its regulation to tissue homeostasis.
Beyond muscular dystrophy, FRG1 has been implicated in angiogenesis and vascular biology. Research with FRG1 antibody has revealed that it influences endothelial cell migration and capillary formation. Dysregulation of FRG1 may therefore contribute to vascular defects in addition to muscle abnormalities. This broadens the biological significance of FSHD region gene 1 protein to multiple tissues and disease processes.
FRG1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting identifies both endogenous and overexpressed FRG1, immunohistochemistry localizes it in muscle tissue, and immunofluorescence shows colocalization with nuclear speckles and actin filaments. These applications make FRG1 antibody indispensable for muscular dystrophy research.
By supplying validated FRG1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
