| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GABBR2 recombinant protein (Position: D100-Q889) was used as the immunogen for the GABBR2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GABBR2 Antibody / GABA-B receptor 2 is a anti-GABBR2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cell membrane, cytoplasm.
Key elements and design rationale
- Target: GABBR2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
GABBR2 is a transmembrane protein encoded by the GABBR2 gene, located on human chromosome 9q22.33. It contains a large extracellular Venus flytrap domain, a seven-transmembrane GPCR domain, and a long cytoplasmic tail involved in receptor assembly and signaling. The interaction of GABBR2 with GABBR1 is required for proper receptor localization at the cell surface. Dysregulation or mutations in GABBR2 have been associated with neurodevelopmental disorders, such as Rett-like syndrome and epileptic encephalopathy, emphasizing its importance in synaptic regulation. Experimental models have shown that altered GABA-B receptor function contributes to impaired inhibitory signaling and enhanced neuronal hyperexcitability.
The GABBR2 antibody is particularly valuable in immunohistochemistry for mapping GABA-B receptor expression across different brain regions, including the hippocampus, cortex, cerebellum, and spinal cord. Western blot analysis using this antibody often reveals bands around 100-110 kDa, corresponding to the mature receptor subunit. It is also useful in co-immunoprecipitation studies to confirm receptor dimerization with GABBR1 and interaction with downstream signaling molecules such as G-proteins and adenylate cyclases. Because of its high specificity and reproducibility, this antibody has become a standard reagent for characterizing the molecular and cellular mechanisms of inhibitory neurotransmission.
Research into GABBR2 continues to illuminate its roles beyond classical neurotransmission. Studies have linked GABA-B receptor signaling to cognitive flexibility, mood regulation, and drug dependence. Pharmacological modulation of this receptor has shown promise in treating anxiety, spasticity, and addiction disorders. The availability of a reliable GABBR2 antibody facilitates continued exploration into the receptor's signaling mechanisms, post-translational modifications, and potential therapeutic targets in neurological and psychiatric diseases.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
