| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GALNT12 recombinant protein (Position: E49-Q572) was used as the immunogen for the GALNT12 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GALNT12 Antibody / Polypeptide N-acetylgalactosaminyltransferase 12 is a anti-GALNT12 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: GALNT12
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Structurally, GALNT12 contains a catalytic domain typical of the GalNAc-transferase family and a ricin-like lectin domain that mediates substrate recognition. It preferentially glycosylates substrates involved in epithelial integrity and mucin production. GALNT12 is expressed in gastrointestinal tissues and epithelial linings, where it influences mucin biosynthesis, cell adhesion, and signaling. Its activity affects the glycoprotein composition of the extracellular matrix and epithelial surfaces.
The GALNT12 antibody is widely used in glycobiology, cancer, and epithelial research to study O-glycosylation and mucin biosynthesis. Western blot analysis identifies a 60 kilodalton band corresponding to GALNT12, while immunofluorescence reveals perinuclear staining consistent with Golgi localization. This antibody is instrumental in evaluating changes in glycosylation during differentiation, inflammation, and tumorigenesis.
Mutations in GALNT12 are associated with colorectal cancer susceptibility, where altered O-glycosylation patterns affect cell adhesion and immune recognition. Dysregulated GALNT12 expression may lead to incomplete mucin glycosylation, contributing to tumor progression and epithelial dysfunction. The GALNT12 antibody supports research into glycosylation-dependent signaling, epithelial homeostasis, and cancer pathogenesis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
