| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GALNT16 recombinant protein (Position: M1-T558) was used as the immunogen for the GALNT16 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GALNT16 Antibody / Polypeptide N-acetylgalactosaminyltransferase 16 is a anti-GALNT16 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GALNT16
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Structurally, GALNT16 contains a catalytic domain responsible for GalNAc transferase activity and a C-terminal ricin-like lectin domain that enhances substrate recognition. It exhibits unique substrate specificity, preferentially modifying glycoproteins involved in neuronal signaling, epithelial maintenance, and immune responses. GALNT16 localizes to the Golgi apparatus, consistent with its function in post-translational modification and protein trafficking.
The GALNT16 antibody is widely used in glycobiology, cancer, and neurobiology research to investigate O-glycosylation pathways and their role in cellular communication. Western blot analysis identifies a 64 kilodalton band corresponding to GALNT16, while immunofluorescence reveals Golgi-localized perinuclear staining. This antibody provides a valuable tool for studying differential glycosylation patterns and their impact on protein stability and cell signaling.
Altered GALNT16 expression has been observed in cancers, where changes in O-glycosylation influence tumor cell invasion and immune evasion. Its expression in brain tissue also suggests involvement in synaptic regulation and neurodevelopment. The GALNT16 antibody enables exploration of how glycosylation controls key biological processes in health and disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
