| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GALNT2 recombinant protein (Position: H442-Q571) was used as the immunogen for the GALNT2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GALNT2 Antibody / Polypeptide N-acetylgalactosaminyltransferase 2 is a anti-GALNT2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Rat.
Key elements and design rationale
- Target: GALNT2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, ELISA
Biological background
Functionally, GALNT2 antibody identifies a 571-amino-acid type II transmembrane protein with an N-terminal cytoplasmic tail, a single transmembrane helix, and a luminal catalytic domain. GALNT2 controls the first step of O-glycan biosynthesis, generating the Tn antigen precursor used for complex glycan assembly. Its substrate selectivity contributes to tissue-specific glycosylation patterns that affect protein stability, receptor signaling, and extracellular matrix composition. Through this activity, GALNT2 regulates cellular adhesion, immune recognition, and lipid metabolism.
The GALNT2 gene is located on chromosome 1q42.13 and is broadly expressed in liver, pancreas, intestine, and epithelial tissues. Genetic studies link GALNT2 variants to plasma lipid levels and susceptibility to metabolic disorders. Reduced GALNT2 expression impairs O-glycosylation of apolipoprotein C-III (ApoC3) and ANGPTL3, leading to altered triglyceride metabolism. Conversely, overexpression influences cell surface receptor function and proteolytic shedding of glycoproteins involved in inflammation and cancer progression.
In cellular signaling, GALNT2 participates in the regulation of the epidermal growth factor receptor (EGFR) and insulin receptor pathways. Aberrant O-glycosylation mediated by GALNT2 modifies receptor trafficking and ligand responsiveness. In cancer, dysregulated GALNT2 expression is associated with increased proliferation, migration, and metastasis in hepatocellular carcinoma and breast cancer. Conversely, its reduced expression can impair glycan-mediated cell communication and immune regulation.
GALNT2 antibody is used for immunohistochemistry, western blotting, and glycoproteomics studies to assess Golgi function, protein maturation, and post-translational modification patterns. The enzyme's activity is crucial for mucin biosynthesis, glycoprotein secretion, and metabolic balance. In metabolic research, GALNT2 serves as a biomarker linking glycosylation to lipid homeostasis and cardiovascular disease risk.
Structurally, GALNT2 contains a catalytic Gal/GalNAc-T motif and a C-terminal ricin-like lectin domain that determines substrate specificity. These features allow recognition of unmodified peptide regions and extension of O-linked glycans in a sequential manner.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
