| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GALP recombinant protein (Position: M1-S116) was used as the immunogen for the GALP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GALP Antibody / Galanin-like peptide is a anti-GALP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GALP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, GALP antibody identifies a 60-amino-acid peptide derived from a larger precursor encoded by the GALP gene. The peptide binds to galanin receptors GALR1, GALR2, and GALR3, activating G protein-mediated signaling pathways that influence neuronal excitability and neuroendocrine secretion. GALP is expressed primarily in the arcuate nucleus and median eminence of the hypothalamus, where it regulates appetite, body temperature, and pituitary hormone release. Its expression is modulated by leptin, insulin, and circulating glucose levels, positioning GALP as a metabolic signal integrator.
The GALP gene is located on chromosome 19q13.43 and exhibits conserved expression in mammals, reflecting its importance in metabolic control. GALP neurons project to hypothalamic and extrahypothalamic regions involved in feeding and thermoregulation, including the paraventricular nucleus and dorsal raphe.
Pathologically, dysregulation of GALP signaling contributes to metabolic and neuroendocrine disorders such as obesity, infertility, and hypothalamic dysfunction. Reduced GALP expression is associated with leptin deficiency and disrupted reproductive cycling, while elevated levels influence energy expenditure and thermogenesis. Research using GALP antibody supports studies in neuroendocrinology, energy metabolism, and reproductive physiology.
GALP antibody is validated for immunohistochemistry, immunofluorescence, and ELISA to detect neuropeptides in hypothalamic and pituitary tissues.
Structurally, Galanin-like peptide shares sequence homology with galanin in its N-terminal region, allowing interaction with the same receptor family but with distinct potency and distribution. Post-translational processing of prepro-GALP yields the mature peptide with amidated C-terminus essential for receptor activation. This antibody enables analysis of GALP's role in central metabolic control and neuroendocrine integration.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
