| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GAREM1 recombinant protein (Position: Q277-E753) was used as the immunogen for the GAREM1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GAREM1 Antibody / GRB2-associated and regulator of MAPK protein 1 is a anti-GAREM1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: GAREM1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
GAREM1 is encoded by the GAREM1 gene located on human chromosome 18p11.32. The protein is approximately 95 kilodaltons and contains multiple functional regions, including a pleckstrin homology (PH) domain, proline-rich motifs, and SH3-binding sequences that facilitate interaction with growth factor receptor-bound protein 2 (GRB2). Through these interactions, GAREM1 acts as a scaffolding adaptor that transmits extracellular growth factor signals to downstream MAPK/ERK cascades, modulating transcriptional responses and cytoskeletal reorganization.
The GAREM1 antibody detects a 90-100 kilodalton protein on western blot and reveals cytoplasmic and perinuclear localization by immunofluorescence. Activation of EGFR or FGFR promotes tyrosine phosphorylation of GAREM1, which enhances its association with signaling molecules such as SHP2, ERK1/2, and SOS1. Functionally, GAREM1 contributes to cell cycle progression and survival, and its overexpression has been observed in certain cancers, including hepatocellular carcinoma and glioblastoma. Depletion or inhibition of GAREM1 disrupts ERK activation, leading to reduced cell proliferation and migration.
GAREM1 also influences differentiation processes by regulating cytoskeletal dynamics and cell adhesion. In neurons, GAREM1 modulates neurite outgrowth and receptor clustering, linking receptor tyrosine kinase signaling to actin remodeling. It may further act as a regulatory hub integrating MAPK and PI3K signaling pathways.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
