| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GARS1 recombinant protein (Position: E61-D673) was used as the immunogen for the GARS1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GARS1 Antibody / Glycyl-tRNA synthetase is a anti-GARS1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GARS1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, GARS1 antibody identifies a 739-amino-acid enzyme that performs the aminoacylation reaction in two steps: activation of glycine to form glycyl-adenylate, followed by transfer to tRNAGly. This reaction supplies aminoacyl-tRNA for translation elongation, ensuring proper incorporation of glycine into nascent polypeptides. GARS1 operates as a homodimer and belongs to the multi-synthetase complex in higher eukaryotes, coordinating translation efficiency and cellular growth. Its catalytic precision is essential for maintaining proteome integrity and translational accuracy.
The GARS1 gene is located on chromosome 7p15.3 and is ubiquitously expressed in all tissues, reflecting its fundamental role in protein biosynthesis. Mutations in GARS1 cause Charcot-Marie-Tooth disease type 2D (CMT2D) and distal spinal muscular atrophy type V, which are characterized by axonal degeneration and motor neuron dysfunction. These mutations often alter enzyme dimerization or substrate binding, impairing aminoacylation activity and disrupting neuronal protein synthesis.
Beyond its canonical translation function, GARS1 exhibits moonlighting roles in signaling and neuroprotection. It can interact with neuropilin-1 and vascular endothelial growth factor (VEGF) receptors to modulate axonal guidance and angiogenesis. In response to cellular stress, GARS1 may relocate to stress granules, linking protein translation to adaptive stress responses. Overexpression or aggregation of mutant GARS1 variants contributes to neuronal toxicity and mitochondrial dysfunction, highlighting its importance in neurodegenerative disease models.
GARS1 antibody is used in molecular biology and neurobiology research to examine aminoacylation, translation control, and disease-associated mutations. It is suited for immunoblotting, enzyme activity assays, and immunofluorescence studies of cytoplasmic and mitochondrial distribution. In cancer research, increased GARS1 expression has been linked to enhanced translation rates and tumor proliferation. Its detection also provides insights into translational stress responses in metabolic and neurological disorders.
Structurally, GARS1 consists of a catalytic domain containing the active-site lysine responsible for ATP-dependent amino acid activation, a tRNA-binding domain, and a dimerization interface. The enzyme's activity is modulated by phosphorylation and association with other aminoacyl-tRNA synthetases within multi-enzyme assemblies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
