| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GAS7 recombinant protein (Position: M1-Q421) was used as the immunogen for the GAS7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GAS7 Antibody / Growth arrest-specific protein 7 is a anti-GAS7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GAS7
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
GAS7 acts as a structural and regulatory protein linking signaling pathways to cytoskeletal remodeling. Its F-BAR domain promotes membrane tubulation and extension of cellular processes, while its WW domain mediates interaction with proline-rich signaling proteins. GAS7 facilitates neurite initiation and elongation by coordinating actin and microtubule dynamics. In non-neuronal cells, it contributes to growth arrest and differentiation through modulation of cytoskeletal tension. Its expression is upregulated during cell cycle exit and differentiation, suggesting a role in maintaining terminal cell identity.
The GAS7 antibody is used in neurobiology, developmental, and cancer research to detect Growth arrest-specific protein 7 expression and localization. Western blot analysis typically identifies a 48 kilodalton band, while immunofluorescence shows cytoplasmic and membrane-associated staining consistent with its role in actin remodeling. GAS7 is also studied for its involvement in leukemia, where chromosomal translocations involving GAS7 and transcription factors such as EWSR1 have been identified, indicating potential roles in oncogenic fusion gene formation.
Beyond neuronal function, GAS7 is expressed in macrophages and fibroblasts where it regulates cell spreading, migration, and vesicle trafficking. The GAS7 antibody supports research into cytoskeletal signaling and differentiation control, particularly in studies of neuronal morphogenesis and actin network dynamics.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
