| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GATB recombinant protein (Position: R113-E443) was used as the immunogen for the GATB antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GATB Antibody / Glutamyl-tRNA amidotransferase subunit B is a anti-GATB Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: GATB
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, GATB is a 471-amino-acid mitochondrial protein of approximately 52 kilodaltons that contains an amidase domain and ATP-binding sites required for the transamidation process. It associates with the catalytic subunit GATC and the tRNA recognition subunit GATA to form the functional GatCAB complex. Within mitochondria, GATB localizes to the matrix, where it couples amino acid activation with tRNA modification to sustain accurate translation of mitochondrial-encoded proteins.
The GATB antibody is widely used in mitochondrial biology, enzymology, and translation research to study aminoacyl-tRNA synthesis, protein biosynthesis fidelity, and mitochondrial genome maintenance. Western blot analysis detects a 52 kilodalton band corresponding to GATB, while immunofluorescence shows punctate mitochondrial staining that colocalizes with mitochondrial matrix markers such as HSP60. This antibody provides a dependable reagent for monitoring translational control and enzyme complex integrity in mitochondria.
Functionally, GATB ensures translational fidelity by catalyzing the amination of misacylated tRNAs, preventing incorporation of incorrect amino acids during protein synthesis. Loss or mutation of GATB disrupts mitochondrial translation and compromises respiratory chain assembly, leading to mitochondrial dysfunction, energy deficiency, and developmental disorders. Altered expression of GATB has been linked to neurodegenerative conditions and metabolic syndromes associated with impaired mitochondrial translation. The GATB antibody enables mechanistic studies of mitochondrial gene expression, enzyme complex assembly, and translational regulation under physiological and stress conditions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
