| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GATM recombinant protein (Position: M1-E279) was used as the immunogen for the GATM antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GATM Antibody / Glycine amidinotransferase mitochondrial is a anti-GATM Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Mitochondria.
Key elements and design rationale
- Target: GATM
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Functionally, GATM antibody identifies a 423-amino-acid mitochondrial matrix enzyme that catalyzes a reversible transamidination reaction dependent on pyridoxal phosphate (PLP) as a cofactor. This reaction provides the foundation for creatine synthesis, linking amino acid metabolism with cellular energy storage. The enzyme's activity supports energy homeostasis by ensuring adequate creatine production for high-energy phosphate transfer through the phosphocreatine system.
The GATM gene is located on chromosome 15q21.1 and encodes a homotetrameric enzyme targeted to mitochondria via an N-terminal transit peptide. GATM is primarily expressed in kidney, pancreas, and liver, where it supplies guanidinoacetate for systemic creatine synthesis. In muscle and neural tissues, creatine generated downstream of GATM activity is phosphorylated by creatine kinase to form phosphocreatine, maintaining ATP levels during high metabolic activity. Deficiency or mutation of GATM causes creatine deficiency syndrome, characterized by neurological impairment and muscle weakness.
GATM antibody is widely used in studies of amino acid metabolism, energy homeostasis, and mitochondrial function. It is a valuable marker for assessing creatine biosynthesis and mitochondrial enzyme integrity. Reduced GATM activity is linked to metabolic and neurological disorders, while upregulation has been observed under energy stress and oxidative conditions. The enzyme's function connects arginine metabolism, urea cycle intermediates, and ATP regeneration.
Structurally, GATM consists of an active-site lysine residue forming a Schiff base with PLP, enabling catalysis of amidino transfer. The enzyme forms stable homotetramers that ensure efficient substrate channeling and regulation. GATM activity is modulated by substrate availability and feedback inhibition from creatine levels. It interacts with mitochondrial transport proteins to coordinate substrate exchange across the inner membrane. A GATM antibody supports research applications including western blotting, immunohistochemistry, and metabolic flux analysis, providing insights into energy metabolism and mitochondrial health.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
