| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GBA2 recombinant protein (Position: E17-Q869) was used as the immunogen for the GBA2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GBA2 Antibody / Non-lysosomal glucosylceramidase is a anti-GBA2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GBA2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, GBA2 antibody identifies a 927-amino-acid membrane-associated protein localized to the endoplasmic reticulum and Golgi apparatus. GBA2 catalyzes the removal of glucose from glucosylceramide, complementing the lysosomal enzyme GBA1 but functioning in non-lysosomal compartments. By regulating ceramide and glucosylceramide levels, GBA2 affects cell differentiation, apoptosis, and neuronal lipid metabolism.
The GBA2 gene is located on chromosome 9p13.3 and is highly expressed in brain, liver, testis, and kidney. Its activity is essential for maintaining sphingolipid turnover and membrane lipid composition, particularly in neuronal and reproductive tissues. GBA2 functions as a key metabolic enzyme in glycosphingolipid homeostasis and intracellular lipid transport.
Pathologically, mutations in GBA2 cause hereditary spastic paraplegia type 46 and autosomal recessive cerebellar ataxia, disorders characterized by impaired motor function and cerebellar degeneration. Loss of GBA2 activity leads to accumulation of glucosylceramide, resulting in disrupted lipid trafficking and neuronal dysfunction. Research using GBA2 antibody supports studies in lipid metabolism, neurobiology, and metabolic disease mechanisms.
GBA2 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect glucosylceramidase enzymes.
Structurally, Non-lysosomal glucosylceramidase belongs to the glycoside hydrolase family 116 and contains a catalytic domain with a conserved nucleophilic residue required for glucosyl transfer. The enzyme is anchored to membranes through hydrophobic regions and operates in concert with lipid transporters. This antibody facilitates investigation of GBA2's role in non-lysosomal glycosphingolipid metabolism and neurological function.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
