| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GBF1 recombinant protein (Position: E110-K1600) was used as the immunogen for the GBF1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GBF1 Antibody / Golgi-specific brefeldin A-resistance factor 1 is a anti-GBF1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Golgi.
Key elements and design rationale
- Target: GBF1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Functionally, GBF1 antibody identifies a 1859-amino-acid protein that catalyzes GDP-GTP exchange on ARF1, ARF3, and related GTPases, stimulating COPI-coated vesicle assembly and Golgi-to-ER transport. GBF1 localizes to the cis-Golgi and early endosomes, where it regulates membrane curvature and vesicle budding. Brefeldin A inhibits many GEFs but not GBF1, making it a critical regulator of Golgi architecture during stress or drug treatment.
The GBF1 gene is located on chromosome 10q24.32 and is ubiquitously expressed in mammalian cells, with high abundance in secretory tissues. GBF1 coordinates with its effectors, such as BIG1 and BIG2, to sustain bidirectional membrane traffic between the endoplasmic reticulum and Golgi complex. Its activity is modulated by lipid composition, phosphorylation, and interaction with small GTPases and coatomer complexes.
Pathologically, GBF1 dysfunction disrupts vesicular trafficking and contributes to Golgi fragmentation, neurodegeneration, and viral replication. Several RNA viruses exploit GBF1 to remodel host membranes for replication complexes. Conversely, depletion of GBF1 can impair secretion and protein glycosylation, leading to ER stress and altered cellular homeostasis.
GBF1 antibody is widely used in cell biology and virology research to study Golgi dynamics, ARF signaling, and vesicle trafficking. It is suitable for western blotting, immunofluorescence, and immunoprecipitation.
Structurally, GBF1 contains a Sec7 catalytic domain responsible for nucleotide exchange, flanked by dimerization and regulatory domains that ensure specificity for ARF family substrates. Its structural organization supports dynamic localization to Golgi membranes and adaptation to cellular conditions. This antibody enables detailed study of Golgi organization, secretory pathway regulation, and virus-host interactions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
