| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GCH1 recombinant protein (Position: M1-S250) was used as the immunogen for the GCH1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GCH1 Antibody / GTP cyclohydrolase 1 is a anti-GCH1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GCH1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
GCH1 antibody applications extend across neuroscience, cardiovascular research, and metabolic disorders. BH4 deficiency due to mutations in GCH1 results in disorders such as dopa-responsive dystonia (Segawa's disease) and hyperphenylalaninemia. GCH1 expression is tightly regulated by inflammatory signals, oxidative stress, and cytokines such as TNF-alpha and IFN-gamma. In endothelial cells, GCH1 controls endothelial nitric oxide synthase (eNOS) coupling and NO production, influencing vascular tone, blood pressure, and oxidative balance. Dysregulation or polymorphisms of GCH1 have been associated with hypertension, diabetes, and neuropathic pain syndromes.
At the cellular level, GCH1 antibody helps characterize tissue-specific expression patterns and study enzyme regulation in neuronal, hepatic, and vascular tissues. The GCH1 enzyme functions as a homodecamer, with allosteric regulation mediated by the feedback regulatory protein GFRP (GTP cyclohydrolase I feedback regulatory protein). Together, GCH1 and GFRP form a complex controlling BH4 biosynthesis according to phenylalanine and BH4 levels. GCH1 is encoded by the GCH1 gene located on chromosome 14q22.1-q22.2, producing a 250-amino acid cytosolic protein. Mutations include both autosomal dominant and recessive variants leading to enzymatic dysfunction and neurotransmitter deficiency syndromes.
Beyond metabolic function, GCH1 expression increases in response to oxidative stress and acts as a cellular protector by enhancing antioxidant capacity. Overexpression can reduce reactive oxygen species, while silencing sensitizes cells to oxidative damage.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
