| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GCLC recombinant protein (Position: R24-H388) was used as the immunogen for the GCLC antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GCLC Antibody / Glutamate-cysteine ligase catalytic subunit is a anti-GCLC Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: GCLC
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, WB
Biological background
Structurally, GCLC is a 73 kDa cytosolic enzyme that functions as the catalytic component of the heterodimeric glutamate-cysteine ligase complex, together with the modifier subunit GCLM. It contains ATP-binding and substrate-recognition domains that mediate the ligation reaction. GCLC belongs to the ATP-dependent ligase family and serves as the principal control point in GSH biosynthesis. Co-localization studies show cytoplasmic distribution in metabolically active tissues, aligning with its antioxidant role.
Functionally, GCLC maintains cellular antioxidant defenses by providing the precursor for glutathione synthesis. Glutathione serves as a cofactor for numerous detoxification enzymes, including glutathione peroxidases and glutathione S-transferases. Through GSH production, GCLC supports redox regulation, protein thiol homeostasis, and protection against electrophilic stress. In immune cells, GCLC-derived glutathione regulates T-cell activation and macrophage inflammatory responses. Known substrates include L-glutamate, L-cysteine, and ATP.
Deficiency or dysregulation of GCLC results in decreased glutathione levels, leading to oxidative stress, hemolytic anemia, and liver dysfunction. Mutations in GCLC are associated with glutathione synthetase deficiency and neurological disorders linked to oxidative damage. Overexpression is observed in certain cancers, conferring chemoresistance through enhanced antioxidant capacity. Pathway associations include glutathione metabolism, oxidative stress response, and xenobiotic detoxification. During development, GCLC supports organogenesis by protecting proliferating cells from oxidative injury.
The GCLC antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
