| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GCNT2 recombinant protein (Position: E27-Q397) was used as the immunogen for the GCNT2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GCNT2 Antibody / Beta-1,6-N-acetylglucosaminyltransferase 2 is a anti-GCNT2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Golgi).
Key elements and design rationale
- Target: GCNT2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IF, FACS, ELISA
Biological background
GCNT2 functions by transferring N-acetylglucosamine residues via a beta1,6 linkage to galactose units, generating branched carbohydrate structures. These modifications affect protein stability, receptor signaling, and cell-cell interactions. The enzyme has three transcript isoforms (A, B, and C), each exhibiting tissue-specific expression patterns and promoter usage. GCNT2A is predominant in erythroid cells, while GCNT2B and GCNT2C are expressed in non-hematopoietic tissues such as the lens and gastrointestinal tract.
Structurally, GCNT2 contains a conserved catalytic domain typical of glycosyltransferases, including the DXH motif required for donor sugar binding. It is anchored in the Golgi membrane by a short N-terminal transmembrane domain, positioning its catalytic site within the lumen for glycan modification. GCNT2 belongs to the GT14 family of glycosyltransferases, which also includes GCNT1 and GCNT3, enzymes responsible for branching of mucin-type O-glycans and other carbohydrate chains.
Functionally, GCNT2 is responsible for converting linear i antigens into branched I antigens during erythrocyte maturation. Loss of GCNT2 activity results in the adult i blood group phenotype, characterized by persistence of fetal-type linear glycans. In the lens, GCNT2 participates in glycoprotein processing required for lens transparency, while in epithelial tissues, it regulates mucin glycosylation that influences cell adhesion and immune defense. Known substrates of GCNT2 include glycoproteins bearing poly-N-acetyllactosamine extensions such as laminin and selectins.
Mutations in GCNT2 are associated with congenital cataracts and the rare adult i blood group phenotype. Reduced enzymatic activity leads to accumulation of unbranched glycan structures, affecting membrane organization and cell surface recognition. In cancer, altered GCNT2 expression has been observed in breast, colon, and gastric carcinomas, where changes in glycan branching influence cell motility and metastasis. Pathway associations include glycosphingolipid biosynthesis, protein glycosylation, and immune recognition processes.
Immunohistochemical staining using GCNT2 antibody shows Golgi localization in epithelial and erythroid cells. The GCNT2 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
