| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse Gdf15 recombinant protein (Position: S189-A302) was used as the immunogen for the Gdf15 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Gdf15 Antibody / Growth differentiation factor 15 is a anti-GDF15 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: GDF15
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
GDF15 is synthesized as a precursor protein that undergoes proteolytic cleavage to release a mature disulfide-linked dimer. It exerts its biological effects primarily through binding to the GDNF family receptor alpha-like (GFRAL) receptor in the brainstem, which, together with RET co-receptor, mediates appetite suppression and energy expenditure. In peripheral tissues, GDF15 influences macrophage activation, anti-inflammatory responses, and tissue protection under stress. Co-localization studies show GDF15 present in secretory vesicles of hepatocytes and trophoblasts, as well as circulating in plasma as a stable cytokine dimer.
Structurally, GDF15 shares conserved cysteine residues and dimerization motifs characteristic of the transforming growth factor beta family. It forms a homodimeric structure essential for receptor binding and downstream signaling activation. GDF15 belongs to the growth differentiation factor family, which includes GDF11, GDF8 (myostatin), and GDF9, all of which regulate growth and differentiation processes. Known interacting partners include GFRAL, RET, and transforming growth factor beta receptors in certain contexts.
Functionally, GDF15 acts as a key regulator of systemic metabolism and cellular stress response. It suppresses appetite and body weight through the GFRAL-RET signaling axis in the central nervous system while also promoting tissue tolerance to metabolic stress. In macrophages and endothelial cells, GDF15 has anti-inflammatory effects, limiting excessive immune activation. In the cardiovascular system, GDF15 protects against ischemic injury by reducing apoptosis and oxidative stress. During embryonic development, it is expressed in placenta and plays roles in implantation and trophoblast differentiation.
Dysregulation of GDF15 expression is associated with numerous pathological conditions. Elevated circulating levels occur in cancer, obesity, cardiovascular disease, and mitochondrial disorders, where GDF15 serves as a biomarker of cellular stress. In oncology, high GDF15 promotes cachexia and tumor progression via immune and metabolic pathways. Conversely, GDF15 has protective effects in metabolic diseases by improving glucose tolerance and reducing inflammation. Pathway involvement includes transforming growth factor beta signaling, mitochondrial stress response, and energy metabolism regulation.
Immunohistochemical staining using GDF15 antibody shows cytoplasmic and extracellular localization in hepatocytes, macrophages, and placental trophoblasts. The GDF15 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
