| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GGA2 recombinant protein (Position: L26-A608) was used as the immunogen for the GGA2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GGA2 Antibody / Golgi-localized, gamma ear-containing, ARF-binding protein 2 is a anti-GGA2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: GGA2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IP, WB
Biological background
Structurally, GGA2 is a 613-amino-acid cytoplasmic protein of approximately 70 kilodaltons composed of three major domains: a VHS (Vps27, Hrs, STAM) domain that recognizes sorting signals on cargo proteins, a GAT (GGA and TOM1) domain that binds ARF1-GTP, and a GAE (gamma-adaptin ear) domain that recruits accessory proteins involved in vesicle formation. These domains coordinate to promote cargo capture, clathrin coat assembly, and vesicle budding from the TGN.
The GGA2 antibody is widely used in cell biology, membrane trafficking, and protein transport research to study endosomal sorting, Golgi organization, and receptor recycling. Western blot analysis detects a 70 kilodalton band corresponding to GGA2, while immunofluorescence reveals punctate perinuclear staining consistent with Golgi localization. This antibody serves as a valuable tool for examining vesicle-mediated transport and adaptor protein function in secretory pathways.
Functionally, GGA2 interacts with mannose-6-phosphate receptors and lysosomal hydrolase precursors, mediating their trafficking to late endosomes and lysosomes. It also contributes to receptor downregulation and recycling through ARF-dependent membrane dynamics. Loss or disruption of GGA2 impairs lysosomal enzyme delivery and can cause abnormal accumulation of cargo proteins within the Golgi apparatus. The GGA2 antibody enables detailed study of Golgi-to-endosome transport, cargo recognition mechanisms, and clathrin coat assembly.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
