| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GGCT recombinant protein (Position: N25-L188) was used as the immunogen for the GGCT antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GGCT Antibody / Gamma-glutamylcyclotransferase is a anti-GGCT Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GGCT
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Gamma-glutamylcyclotransferase localizes primarily to the cytosol, where it regulates the degradation of glutathione conjugates. By catalyzing gamma-glutamyl bond cleavage, the enzyme supports the recycling of amino acids used for glutathione synthesis. The GGCT antibody enables visualization and quantification of this enzyme in cells and tissues, supporting research into oxidative stress–related pathways and cellular detoxification processes. Through its activity, GGCT contributes to maintaining redox homeostasis and protecting against reactive oxygen species (ROS) accumulation.
Upregulation of Gamma-glutamylcyclotransferase has been reported in various cancers, including breast, lung, and bladder tumors, where it is associated with enhanced proliferation and stress resistance. Elevated GGCT expression supports tumor growth by maintaining redox stability and promoting metabolic flexibility. The GGCT antibody is used to investigate these oncogenic functions, providing insight into how altered glutathione metabolism contributes to tumorigenesis. It also enables assessment of GGCT as a potential biomarker or therapeutic target in oxidative stress–driven cancers.
Beyond cancer, Gamma-glutamylcyclotransferase plays roles in neuroprotection and metabolic adaptation. Its regulation affects glutathione turnover in the brain, liver, and kidneys, influencing detoxification capacity and antioxidant defense. The GGCT antibody allows researchers to track expression changes in response to nutritional status, xenobiotic exposure, and oxidative injury. Dysregulation of GGCT may contribute to neurodegenerative diseases and metabolic disorders characterized by redox imbalance.
The GGCT antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing strong cytoplasmic staining consistent with its enzymatic localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
