| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Gamma glutamyl hydrolase was used as the immunogen for the GGH antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GGH Antibody / Gamma glutamyl hydrolase is a anti-GGH Rabbit antibody Recombinant Rabbit Monoclonal clone 31G17 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: GGH
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31G17, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
GGH antibody is widely applied in oncology, pharmacology, and metabolism research. Elevated GGH expression reduces the retention and potency of antifolate drugs, contributing to chemoresistance in leukemia and solid tumors. Conversely, altered GGH activity affects folate availability and one carbon metabolism, impacting DNA synthesis and repair. By detecting GGH, researchers can study how folate metabolism intersects with cancer biology, drug resistance, and metabolic disease.
Western blot assays detect GGH protein bands in liver and tumor extracts. Immunohistochemistry maps expression in proliferative tissues and tumors, while immunofluorescence highlights cytoplasmic localization in cells actively synthesizing DNA. These approaches provide insight into how GGH distribution correlates with metabolic activity and drug response.
GGH also plays roles in normal physiology, regulating folate supply for rapidly dividing cells in bone marrow, intestinal epithelium, and developing tissues. Dysregulation of GGH has been associated with megaloblastic anemia, cancer progression, and sensitivity to antifolate therapy. By applying GGH antibody, scientists can explore the balance between folate metabolism and proliferation in both health and disease.
GGH antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
