| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GH1 recombinant protein (Position: Q22-F217) was used as the immunogen for the GH1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GH1 Antibody / Growth Hormone is a anti-GH1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GH1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
GH1 is encoded by the GH1 gene located on human chromosome 17q24.2. The mature protein is approximately 22 kilodaltons and derived from a larger precursor that undergoes signal peptide removal and disulfide bond formation in the endoplasmic reticulum. GH1 is stored in secretory granules and released into circulation in response to hypothalamic growth hormone-releasing hormone (GHRH) and inhibited by somatostatin. It plays major roles in postnatal growth, bone development, and metabolic homeostasis.
The GH1 antibody detects the 22 kilodalton hormone by western blot and shows strong cytoplasmic granule staining in pituitary somatotrophs by immunofluorescence. GH1 binds to its receptor on target tissues, activating JAK2 and STAT5 pathways that induce insulin-like growth factor 1 (IGF1) production in the liver and peripheral tissues. Through IGF1 and direct signaling, GH1 stimulates protein synthesis, lipolysis, and glucose utilization.
Aberrations in GH1 production cause growth disorders, including gigantism and acromegaly from overproduction, or growth hormone deficiency due to pituitary malfunction or genetic mutation. Outside growth regulation, GH1 contributes to immune function, cardiac performance, and metabolic adaptation under stress conditions. Elevated GH1 and IGF1 signaling are also linked to cancer cell proliferation and survival.
Because of its central endocrine function, GH1 serves as a key biomarker in clinical diagnostics and metabolic studies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
