| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PSF2/GINS2 recombinant protein (Position: M1-F185) was used as the immunogen for the GINS2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GINS2 Antibody / GINS protein subunit 2 / PSF2 is a anti-GINS2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: GINS2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, FACS, ELISA
Biological background
Functionally, GINS2 antibody identifies a 204-amino-acid nuclear protein required for the progression of DNA replication forks. GINS2 promotes helicase activation, stabilizes replication machinery, and facilitates the unwinding of DNA strands during S-phase. By interacting with key replication factors including MCM2-7, CDC45, and DNA polymerase alpha, GINS2 ensures accurate and efficient genome duplication. It also contributes to replication checkpoint control, preventing DNA damage accumulation and maintaining genomic stability.
The GINS2 gene is located on chromosome 16q24.1 and is expressed in proliferating tissues such as bone marrow, thymus, and tumors. Expression is cell cycle-regulated, peaking during S-phase when DNA replication is most active. GINS2 is evolutionarily conserved, highlighting its fundamental role in DNA synthesis and cell division.
Pathologically, GINS2 overexpression has been linked to tumorigenesis and poor prognosis in cancers including breast, lung, and gastric carcinoma. Excessive GINS2 activity enhances cell proliferation, while its depletion leads to replication stress, checkpoint activation, and apoptosis. Research using GINS2 antibody supports studies in DNA replication, cell cycle regulation, and cancer biology.
GINS2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect replication complex components.
Structurally, DNA replication complex GINS protein subunit 2 adopts a beta-strand-rich conformation that contributes to the ring-shaped architecture of the GINS complex. This structural organization provides stability and interaction surfaces for other replication proteins. This antibody facilitates detailed investigation of GINS2's mechanistic role in replication fork progression and cancer-related replication stress.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
