| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GINS3 recombinant protein (Position: M1-D216) was used as the immunogen for the GINS3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GINS3 Antibody / GINS protein subunit 3 is a anti-GINS3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: GINS3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Functionally, GINS3 antibody identifies a 223-amino-acid protein that forms part of the heterotetrameric GINS complex along with GINS1 (Sld5), GINS2 (Psf2), and GINS4 (Psf3). The GINS complex interacts with Cdc45 and the MCM2-7 helicase to form the CMG (Cdc45-MCM-GINS) complex, which drives replication fork unwinding and DNA synthesis. GINS3 stabilizes this complex, ensuring efficient replication initiation and fork progression during S phase.
The GINS3 gene is located on chromosome 16q24.1 and encodes a conserved protein expressed in proliferating cells and tissues undergoing rapid growth. GINS3 functions as a scaffold subunit, mediating interactions between GINS partners and replication factors such as DNA polymerase epsilon. Its depletion leads to stalled replication forks, DNA damage accumulation, and activation of checkpoint signaling, highlighting its essential role in genome duplication.
In cell cycle regulation, GINS3 contributes to origin firing and replication timing control. It is recruited to replication origins during early S phase and remains associated with active replication forks. Dysregulation of GINS3 or other CMG components disrupts DNA replication dynamics and can result in replication stress, chromosomal instability, and tumorigenesis. Overexpression of GINS subunits has been observed in cancers characterized by high proliferation rates, including breast, ovarian, and colorectal cancers.
GINS3 antibody is widely used in cell cycle, DNA replication, and cancer research. It is suitable for immunoblotting, immunofluorescence, and chromatin fractionation assays to examine GINS3 expression and complex formation. This antibody supports studies of replication fork mechanics, checkpoint signaling, and DNA damage responses. In oncology, it helps assess replication stress pathways and therapeutic sensitivity to DNA synthesis inhibitors.
Structurally, GINS3 adopts a beta-sheet-rich fold that interfaces with GINS1 and GINS2 to stabilize the quaternary complex. It acts as a bridge linking DNA helicase and polymerase activities at replication forks.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
