| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | E.coli-derived human GIPC3 recombinant protein (Position: H45-G312) was used as the immunogen for the GIPC3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GIPC3 Antibody / PDZ domain-containing protein GIPC3 / C19orf64 is a anti-GIPC3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Rat.
Key elements and design rationale
- Target: GIPC3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, ICC, WB
Biological background
GIPC3 is encoded by the GIPC3 gene located on human chromosome 19p13.3. The protein is approximately 36 kilodaltons and contains a central PDZ domain that binds C-terminal motifs of target proteins, as well as N- and C-terminal regions that mediate dimerization and association with myosin VI. Through these interactions, GIPC3 organizes membrane trafficking complexes that control receptor recycling and vesicle transport along actin filaments.
The GIPC3 antibody detects a 36 kilodalton band by western blot and shows cytoplasmic punctate localization in sensory epithelial cells. In the cochlea, GIPC3 is essential for hair cell synapse formation and auditory neurotransmission. Mutations in GIPC3 cause hereditary nonsyndromic deafness (DFNB72) by disrupting vesicular transport between inner hair cells and spiral ganglion neurons, leading to progressive hearing loss.
Functionally, GIPC3 modulates receptor signaling pathways, including those mediated by TGF-beta and IGF1, by regulating receptor endocytosis and recycling. It also interacts with proteins such as APPL1 and neuropilin, influencing cell migration and survival signaling. In neurons, GIPC3 may participate in axonal transport and synaptic vesicle cycling.
Beyond the auditory system, altered expression of GIPC3 has been linked to tumor progression and metastasis through modulation of growth factor receptor trafficking. Its PDZ-mediated interactions are targets for potential therapeutic inhibition in cancer and neurodegenerative disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
