| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GKAP1 recombinant protein (Position: E69-R366) was used as the immunogen for the GKAP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GKAP1 Antibody / G kinase-anchoring protein 1 is a anti-GKAP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GKAP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, WB
Biological background
GKAP1 is encoded by the GKAP1 gene located on human chromosome 9q31.1. The protein is approximately 96 kilodaltons and characterized by multiple ankyrin repeats and leucine zipper motifs that mediate protein-protein interactions. It functions as an anchoring protein for PKG, positioning the kinase in proximity to its substrates at specific subcellular compartments, including the cytoskeleton and plasma membrane. This compartmentalization ensures spatial precision in cGMP-mediated signaling events.
The GKAP1 antibody detects a 96 kilodalton band by western blot and shows cytoplasmic and perinuclear localization under immunofluorescence. GKAP1 binds directly to PKG I and II isoforms, facilitating phosphorylation of target proteins involved in smooth muscle relaxation, ion channel regulation, and metabolic control. Through its anchoring role, GKAP1 contributes to compartmentalized cGMP signaling, distinguishing localized kinase activity from global cyclic nucleotide effects.
Beyond its role in vascular signaling, GKAP1 participates in neuronal signaling and synaptic plasticity. It interacts with cytoskeletal proteins and actin-regulatory molecules, influencing axon growth and dendritic morphology. GKAP1 may also modulate insulin signaling and energy metabolism through PKG-mediated pathways in adipocytes and hepatocytes.
Dysregulation of GKAP1 expression alters cGMP signaling fidelity and is associated with cardiovascular disorders, hypertension, and metabolic diseases. Overexpression enhances PKG activity and smooth muscle relaxation, whereas loss of GKAP1 results in impaired vasodilation and reduced cellular responsiveness to nitric oxide. Because of its central role in cGMP signaling scaffolding, GKAP1 represents a valuable target for understanding kinase compartmentalization and cyclic nucleotide signaling networks.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
