| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GKN1 recombinant protein (Position: M1-N185) was used as the immunogen for the GKN1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GKN1 Antibody / Gastrokine 1 is a anti-GKN1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse. Reported localization: Cytoplasm, Golgi, secreted.
Key elements and design rationale
- Target: GKN1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
GKN1 expression is restricted to normal gastric mucosa and is markedly downregulated or lost in gastric cancer, making it a useful biomarker for gastric differentiation and neoplastic transformation. Functional studies demonstrate that GKN1 suppresses gastric tumor cell proliferation, modulates NF-kB signaling, and inhibits epithelial-mesenchymal transition. Loss of GKN1 leads to increased susceptibility to inflammation-induced damage and carcinogenesis. By promoting mucosal healing and preserving epithelial barrier integrity, GKN1 functions as a tumor suppressor and protective factor in the gastrointestinal tract.
The GKN1 antibody is widely used in gastrointestinal biology, cancer, and inflammation research to detect Gastrokine-1 in tissue sections or cell lysates. In immunohistochemistry, GKN1 localizes predominantly to the cytoplasm and secretory granules of surface mucous cells. Western blotting identifies a 19 kilodalton band corresponding to the mature secreted form. The antibody assists in distinguishing normal gastric tissue from malignant lesions, as GKN1 expression is largely absent in gastric adenocarcinoma but retained in non-neoplastic mucosa. This differential expression pattern has diagnostic and prognostic implications.
Beyond the stomach, low levels of GKN1 are occasionally detected in duodenal and pancreatic tissues, suggesting secondary protective roles in the upper gastrointestinal tract. The protein also exhibits antibacterial activity and modulates host responses to Helicobacter pylori infection. Because of these diverse functions, the GKN1 antibody supports research into epithelial regeneration, cancer suppression, and gastric disease pathogenesis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
