| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GLG1 recombinant protein (Position: E173-K1040) was used as the immunogen for the GLG1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GLG1 Antibody / Golgi apparatus protein 1 is a anti-GLG1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GLG1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, GLG1 antibody identifies a 1,474-amino-acid type I membrane glycoprotein containing multiple FG (phenylalanine-glycine)-like and epidermal growth factor (EGF)-like repeats. GLG1 acts as a recycling receptor in the Golgi, binding fibroblast growth factors (FGFs) and contributing to their intracellular trafficking. It also functions as a ligand for E-selectin, mediating leukocyte adhesion to endothelial cells during inflammation. Within the Golgi, GLG1 organizes cisternal structure and assists in the retrieval of escaped proteins from post-Golgi compartments.
The GLG1 gene is located on chromosome 16q22.1 and is widely expressed in epithelial, endothelial, and secretory tissues. Its expression is tightly regulated by growth factors and stress signals, ensuring efficient vesicular transport and glycoprotein processing.
Pathologically, GLG1 dysregulation has been linked to cancer progression, congenital disorders of glycosylation, and inflammatory diseases. Overexpression promotes tumor cell migration and metastasis by enhancing adhesion to E-selectin and modulating FGF signaling. Conversely, reduced GLG1 disrupts protein trafficking and Golgi integrity. Research using GLG1 antibody supports studies in vesicular transport, cell adhesion, and tumor biology.
GLG1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect Golgi-associated proteins.
Structurally, Golgi apparatus protein 1 contains a long luminal region with repetitive domains, a single transmembrane helix, and a short cytoplasmic tail responsible for Golgi retention. The protein's modular structure supports both structural and signaling functions within secretory pathways. This antibody enables exploration of GLG1's role in Golgi function, protein sorting, and inflammatory cell adhesion.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
