| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GLIPR2 recombinant protein (Position: M1-K154) was used as the immunogen for the GLIPR2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GLIPR2 Antibody / GLI pathogenesis-related protein 2 is a anti-GLIPR2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: GLIPR2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IP, IHC, WB
Biological background
Functionally, GLIPR2 antibody recognizes a 172-amino-acid protein that plays a dual role in regulating autophagy and cellular differentiation. GLIPR2 interacts with Beclin-1, a central component of the autophagy initiation complex, to modulate autophagosome formation. Overexpression of GLIPR2 inhibits autophagic flux, whereas its depletion enhances autophagy under stress conditions. This regulatory balance links GLIPR2 to processes such as tumor suppression, cell death, and immune modulation. The protein is expressed in multiple tissues, including liver, kidney, and lung, and its expression is inducible under oxidative or endoplasmic reticulum stress.
The GLIPR2 gene is located on chromosome 9p13.3 and encodes a secreted and membrane-associated protein characterized by a conserved CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1) domain. This structural motif enables interactions with lipids, membranes, and signaling proteins, mediating responses to cellular stress. GLIPR2 is known to form homodimers and is subject to regulation by cholesterol content in the Golgi membrane. This localization allows it to influence vesicle trafficking and protein processing under metabolic challenge.
GLIPR2 antibody is widely used in research investigating autophagy, lipid homeostasis, and tumor biology. In cancer studies, GLIPR2 functions as a modulator of epithelial-to-mesenchymal transition (EMT), influencing cell adhesion, migration, and invasion. Dysregulated GLIPR2 expression has been linked to aggressive tumor phenotypes, including hepatocellular carcinoma and renal cell carcinoma. In non-cancer contexts, GLIPR2 participates in innate immune regulation and Golgi stress adaptation. It also contributes to lung tissue integrity by modulating surfactant secretion and inflammatory signaling.
At the molecular level, GLIPR2 interacts with proteins involved in oxidative stress response and chaperone-assisted folding. Its CAP domain facilitates binding to phosphatidylinositol lipids, anchoring it to specific membrane compartments. The GLIPR2 antibody is an essential tool for detecting this protein in cell biology, immunofluorescence, and immunoblotting applications. Its detection assists in understanding Golgi function, autophagy balance, and membrane organization under normal and pathological conditions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
