| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GLMN recombinant protein (Position: Q153-R574) was used as the immunogen for the GLMN antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GLMN Antibody / Glomulin is a anti-GLMN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GLMN
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Glomulin interacts with Cullin-RING ligases and the SKP1-CUL1-F-box (SCF) complex, regulating substrate ubiquitination and degradation. By controlling ubiquitin homeostasis, it helps maintain cellular proteostasis and vessel wall stability. GLMN localizes to the cytoplasm and perinuclear regions, consistent with its role in ubiquitin regulation and protein quality control.
The GLMN antibody is used in vascular biology, developmental, and protein degradation research to investigate ubiquitination processes, angiogenesis, and smooth muscle differentiation. Western blot analysis identifies a 68 kilodalton band corresponding to Glomulin, while immunofluorescence shows cytoplasmic and perinuclear localization in endothelial and fibroblast cells. This antibody supports the study of vascular signaling and ubiquitin regulation mechanisms.
Mutations in GLMN cause glomuvenous malformations (glomangiomas), benign vascular lesions characterized by aberrant smooth muscle proliferation due to disrupted ubiquitin signaling. GLMN also participates in cell cycle regulation and differentiation, linking protein degradation to tissue morphogenesis. The GLMN antibody provides a robust reagent for studying ubiquitin-related vascular disorders and developmental signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
