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Acetylation of histone H3 at lysine 9 (H3K9) is a post-translational modification where an acetyl group is added to the lysine residue at position 9 on the histone H3 protein. This modification is mediated by histone acetyltransferases (HATs), which add acetyl groups, and histone deacetylases (HDACs), which remove them. H3K9 acetylation is associated with gene activation. It is often found at active promoters and enhancers, facilitating a more open chromatin structure that allows transcription factors to access DNA. Altered acetylation levels at H3K9 have been linked to various cancers, affecting tumor progression and metastasis. The Global Acetyl Histone H3K9 Quantification Kit is designed to measure global histone H3K9 acetylation in various mammalian cells, including human and mouse cells, as well as fresh and frozen tissues, cultured adherent cells, and suspension cells. This assay is simple, user-friendly, accurate, and fast. The measurement can be completed in 2.5 hours, with a recommended sample input of 20 ng to 5 µg per well. Alternative Name: H3K9ac; Acetyl Histone H3K9; Acetylation of histone H3 at lysine 9
Alternative names: H3K9ac; Acetyl Histone H3K9; Acetylation of histone H3 at lysine 9
Assay Principle
The Global Acetyl Histone H3K9 Quantification Kit (Colorimetric) is a colorimetric immunoassay for the quantitative measurement of acetyl-histone h3k9 in mammalian cells and tissues. Target histones are captured on a pre-coated microplate and detected with a modification-specific antibody, then quantified against a standard curve. The assay measures global histone h3k9 acetylation with a recommended sample input of 20 ng – 5 µg per well and can be completed in 2.5 hours.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (modification-specific antibody and enzyme/fluorophore conjugate), wash buffer, and developing/stop solutions. Refer to the product datasheet for the full component list and volumes.
Performance Data
| Assay Target | Global histone H3K9 acetylation |
|---|---|
| Analyte | Acetyl-Histone H3K9 (H3K9ac) |
| Assay Type | Colorimetric immunoassay |
| Detection Method | Colorimetric |
| Assay Time | 2.5 hours |
| Recommended Sample Input | 20 ng – 5 µg per well |
| Organism | Human, Mouse |
| Sample Compatibility | Mammalian cells (human, mouse); fresh & frozen tissues; adherent & suspension cells |
| Storage | Store the unopened kit at 2–8 °C. |
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this global histone h3k9 acetylation assay — including colorimetric or alternative detection readout, plate/pack size (48 or 96-assay microplate), bulk packaging, or a validated protocol for your sample type (cells, tissue, adherent or suspension cultures). Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in contact shortly.
