| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Product Type | |
| Storage |
H3K4 methylation refers to the addition of methyl groups to the lysine 4 residue of histone H3, a process that can occur in three states: mono-, di-, and trimethylation (H3K4me1, H3K4me2, and H3K4me3). This modification is associated with transcriptional activation and is typically found at the promoter and enhancer regions of actively transcribed genes. H3K4 methylation is a conserved chromatin modification across various organisms, from yeast to humans. H3K4 methylation plays a critical role in gene expression regulation, chromatin structure, and various biological processes, including development and differentiation. The Global Histone H3K4 Methylation Assay is designed to measure H3-K4 methylation in various mammalian cells, including human and mouse cells, as well as fresh and frozen tissues, cultured adherent cells, and suspension cells. This assay is simple, accurate, and fast. The measurement can be completed in 5 hours, with a recommended sample input of 5 ng to 2 µg per well.
Assay Principle
The Global Histone H3K4 Methylation Assay Kit is a immunoassay for the quantitative measurement of methylated histone h3k4 in mammalian cells and tissues. Target histones are captured on a pre-coated microplate and detected with a modification-specific antibody, then quantified against a standard curve. The assay measures global histone h3k4 methylation with a recommended sample input of 5 ng – 2 µg per well and can be completed in 5 hours.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (modification-specific antibody and enzyme/fluorophore conjugate), wash buffer, and developing/stop solutions. Refer to the product datasheet for the full component list and volumes.
Performance Data
| Assay Target | Global histone H3K4 methylation |
|---|---|
| Analyte | Methylated Histone H3K4 |
| Assay Type | Immunosorbent assay |
| Detection Method | — |
| Assay Time | 5 hours |
| Recommended Sample Input | 5 ng – 2 µg per well |
| Organism | Human, Mouse |
| Sample Compatibility | Mammalian cells (human, mouse); fresh & frozen tissues; adherent & suspension cells |
| Storage | Store the unopened kit at 2–8 °C. |
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this global histone h3k4 methylation assay — including the required or alternative detection readout, plate/pack size (48 or 96-assay microplate), bulk packaging, or a validated protocol for your sample type (cells, tissue, adherent or suspension cultures). Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in contact shortly.
