Global Tri-Methyl Histone H3K4 Quantification Kit (Colorimetric)

Tri-Methyl Histone H3K4 refers to the trimethylation of the lysine 4 residue on histone H3. H3K4 tri-methylation has been viewed as a signature mark of highly transcribed genes, which is placed exclusively in the 5’- region downstream of the promoter. Increased global H3K4 tri-methylation is also found to be involved in some pathological processes such as cancer progression. Therefore, quantitative detection of global tri-methyl histone H3K4 would provide useful information for better understanding epigenetic regulation of gene activation, and for developing HMT-targeted drugs. The Global Tri-Methyl H3K4 Quantification Kit is designed to measure global histone H3K4 tri-methylation in various mammalian cells, including human and mouse cells, as well as fresh and frozen tissues, cultured adherent cells, and suspension cells. This assay is simple, accurate, and fast. The measurement can be completed in 2.5 hours, with a recommended sample input of 20 ng to 5 µg per well. Standard Preparation

Assay Principle

The Global Tri-Methyl Histone H3K4 Quantification Kit (Colorimetric) is a colorimetric immunoassay for the quantitative measurement of tri-methyl histone h3k4 in mammalian cells and tissues. Target histones are captured on a pre-coated microplate and detected with a modification-specific antibody, then quantified against a standard curve. The assay measures global histone h3k4 trimethylation with a recommended sample input of 20 ng – 5 µg per well and can be completed in 2.5 hours.

Kit Components

The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (modification-specific antibody and enzyme/fluorophore conjugate), wash buffer, and developing/stop solutions. Refer to the product datasheet for the full component list and volumes.

Performance Data

Safety & Handling