| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GLOD4 recombinant protein (Position: E57-G313) was used as the immunogen for the GLOD4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GLOD4 Antibody / Glyoxalase domain-containing protein 4 is a anti-GLOD4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GLOD4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, GLOD4 is a ~36 kDa protein with a glyoxalase-like fold. It is predicted to bind metal ions that assist catalysis, though definitive enzymatic activity has yet to be confirmed. Localization studies suggest enrichment in mitochondria, highlighting a role in redox balance and metabolic regulation.
Functionally, GLOD4 may protect cells from glycation stress by metabolizing reactive aldehydes. Its expression is broad, with higher levels in metabolic tissues such as liver, kidney, and muscle. Researchers use GLOD4 antibody to study mitochondrial metabolism, detoxification pathways, and oxidative stress responses.
Clinically, altered GLOD4 expression has been observed in cancer and metabolic disease. By influencing redox balance, GLOD4 may contribute to tumor survival under stress conditions. It is under investigation as a metabolic biomarker and therapeutic target.
Experimentally, GLOD4 antibody is applied in western blotting to detect the ~36 kDa protein, in immunofluorescence microscopy to study mitochondrial localization, and in immunohistochemistry to assess expression across tissues. Functional studies combining GLOD4 antibody with metabolic assays help clarify its enzymatic role in glyoxalase pathways.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
