| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GLOD5 recombinant protein (Position: M1-S160) was used as the immunogen for the GLOD5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GLOD5 Antibody / Glyoxalase domain-containing protein 5 is a anti-GLOD5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Mitochondria.
Key elements and design rationale
- Target: GLOD5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, GLOD5 antibody recognizes a cytoplasmic protein of approximately 160 amino acids that contains a metalloenzyme fold typical of glyoxalase domains. It is thought to play a role in the metabolism of reactive aldehydes and other cytotoxic intermediates generated during oxidative stress. GLOD5 expression may also contribute to maintaining redox balance and protecting cells from metabolic byproducts.
The GLOD5 gene is located on chromosome 9p13.2 and exhibits widespread expression in human tissues, including brain, kidney, and liver. Its evolutionary conservation among mammals suggests a physiological role in small-molecule metabolism or detoxification pathways. GLOD5 expression has been reported to increase under oxidative stress conditions, further linking it to cellular protection mechanisms.
Pathologically, altered GLOD5 expression may influence cell viability and stress adaptation. Dysregulation of glyoxalase-related enzymes is associated with diabetes, neurodegeneration, and cancer, though the specific contribution of GLOD5 remains under investigation. Using GLOD5 antibody enables detection of this lesser-known member of the glyoxalase family and aids exploration of its potential metabolic roles.
GLOD5 antibody is suitable for western blotting, immunocytochemistry, and immunohistochemistry to detect GLOD5 in cell and tissue extracts.
Structurally, GLOD5 contains a glyoxalase-like catalytic fold capable of coordinating divalent metal ions, although its active-site residues are not fully conserved. This antibody assists researchers in clarifying GLOD5's molecular function and contribution to oxidative metabolism.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
