| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GLUD1 recombinant protein (Position: A210-T558) was used as the immunogen for the GLUD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GLUD1 Antibody / Glutamate dehydrogenase 1 is a anti-GLUD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria, ER).
Key elements and design rationale
- Target: GLUD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IP, IF, IHC, ICC, WB
Biological background
Functionally, GLUD1 antibody identifies a 558-amino-acid enzyme located in the mitochondrial matrix. GLUD1 catalyzes the interconversion of glutamate and alpha-ketoglutarate with the reduction or oxidation of NAD(P)+ cofactors. This reaction controls the flow of carbon and nitrogen through metabolic networks, linking amino acid catabolism, the TCA cycle, and oxidative phosphorylation. In the brain, GLUD1 contributes to the glutamate-glutamine cycle, influencing neurotransmitter turnover and synaptic function.
The GLUD1 gene is located on chromosome 10q23.3 and encodes a hexameric enzyme regulated allosterically by ADP, GTP, leucine, and other metabolites. GLUD1 activity increases during energy demand, providing substrates for ATP synthesis, while its inhibition by GTP prevents excessive ammonia production. Mutations in GLUD1 cause hyperinsulinism-hyperammonemia (HI/HA) syndrome, characterized by dysregulated insulin secretion due to elevated oxidative deamination in pancreatic beta cells.
In hepatic tissue, GLUD1 participates in ammonia detoxification and urea synthesis by controlling the balance between glutamate oxidation and synthesis. In pancreatic islets, its activity modulates insulin release in response to amino acids. In neurons, GLUD1 provides energy intermediates and regulates excitatory neurotransmission. Dysregulation of GLUD1 is implicated in neurodegenerative diseases, metabolic syndromes, and hyperinsulinemic disorders.
GLUD1 antibody is widely used in research involving metabolism, neurobiology, and endocrinology. It is suitable for immunoblotting, immunofluorescence, and enzyme localization studies to assess mitochondrial distribution and activity. This antibody enables detection of GLUD1 in tissues such as liver, brain, and pancreas, where it plays distinct metabolic roles. In cancer research, GLUD1 expression correlates with metabolic reprogramming, supporting tumor cell growth through glutamine oxidation.
Structurally, GLUD1 forms a hexameric complex with alternating catalytic and regulatory domains. The enzyme's activity is modulated by phosphorylation, redox state, and energy charge.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
