| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GNAI2 recombinant protein (Position: R67-N312) was used as the immunogen for the GNAI2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GNAI2 Antibody / Guanine nucleotide-binding protein alpha-2 is a anti-GNAI2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: GNAI2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
GNAI2 is widely expressed in most tissues, including brain, heart, and immune cells, where it plays diverse physiological roles. Upon receptor activation, GNAI2 dissociates from the G beta gamma subunits, allowing each component to activate distinct downstream signaling molecules. Through this mechanism, GNAI2 influences pathways controlling calcium mobilization, ion channel regulation, and MAPK activation. It is particularly important in immune cells, where it mediates chemokine-induced migration and adhesion, and in the nervous system, where it modulates neurotransmitter signaling.
The GNAI2 antibody is widely used in signal transduction, cardiovascular, and neurobiology research. Western blot analysis typically detects a 40 kilodalton band corresponding to the alpha subunit, while immunofluorescence reveals cytoplasmic and membrane localization. In cancer research, altered expression of GNAI2 has been linked to cell proliferation, epithelial-mesenchymal transition, and metastasis. Overexpression or constitutive activation of GNAI2 can promote oncogenic signaling, whereas loss-of-function mutations may disrupt normal G protein signaling dynamics.
Mechanistically, GNAI2 regulates adenylyl cyclase inhibition, phosphatidylinositol 3-kinase (PI3K) activation, and small GTPase modulation. It also interacts with receptor kinases and scaffolding proteins to fine-tune receptor responsiveness. Dysregulation of GNAI2 signaling contributes to cardiovascular dysfunction, immune dysregulation, and tumorigenesis. The GNAI2 antibody enables detailed study of these pathways, supporting analyses of GPCR signaling fidelity and receptor desensitization mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
