| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human GNE was used as the immunogen for the GNE antibody. |
| Isotype | |
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| Target | |
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Overview
GNE Antibody / Glucosamine 6 phosphate N acetyltransferase is a anti-GNE Rabbit antibody Recombinant Rabbit Monoclonal clone 29G96 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.
Key elements and design rationale
- Target: GNE
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29G96, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF
Biological background
GNE antibody is widely applied in metabolic, developmental, and neuromuscular research. Mutations in the GNE gene cause GNE myopathy, also known as hereditary inclusion body myopathy. This rare autosomal recessive disorder is characterized by progressive muscle weakness and degeneration. By using GNE antibody, researchers can investigate enzyme expression levels and localization to better understand the molecular basis of this disease.
The antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, GNE antibody specifically recognizes the protein across tissue samples, confirming its role in metabolic pathways. Immunohistochemistry highlights expression in muscle and epithelial tissues, while immunofluorescence demonstrates cytoplasmic localization within cultured cells. These approaches provide valuable insight into how GNE supports cellular glycosylation and metabolic balance.
In addition to its relevance in genetic disorders, GNE is important in cancer biology and immunology. Altered sialylation patterns are a hallmark of many cancers, where increased sialic acid residues on tumor cells promote immune evasion and metastasis. By monitoring GNE expression with specific antibodies, scientists can explore how dysregulation of sialic acid biosynthesis contributes to malignant transformation. Similarly, in immunology, GNE activity influences immune recognition, inflammation, and infection responses.
Sialic acids play essential roles in development and signaling. Disruption of GNE function affects not only muscle tissue but also systemic processes, highlighting the importance of reliable antibodies for detection. Using GNE antibody, researchers can explore how this key enzyme contributes to glycosylation pathways and how alterations affect health and disease.
GNE antibody offered by
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
