| Field | Specification |
|---|---|
| Clonality | |
| Concentration | |
| Conjugate | |
| Host | |
| Immunogen | Human IgG |
| Isotype | |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
This polyclonal antibody is raised in Goat and targets the target protein. Suitable for Immunofluorescence applications.
Validated Applications & Recommended Dilutions
| Application | Recommended Dilution |
|---|---|
| Immunofluorescence | IF 1:20-100 |
Antibody Specifications
| Target | the target protein |
|---|---|
| Host Species | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | FITC |
| Purification | Affinity Purification |
| Concentration | 0.5 mg/mL |
Immunogen
Human IgG
Species Reactivity & Cross-Reactivity
Validated for: Human. Verify suitability in your sample type prior to use. For species or applications not listed in the datasheet, consult our technical team before purchase.
Storage & Handling
Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Storage Buffer: PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4
Related Products
Browse additional antibodies targeting the target protein and complementary reagents — including secondary antibodies, blocking buffers, and detection kits — available through BioHippo.
For intracellular targets including most nuclear and cytoplasmic proteins, fix cells with 4% paraformaldehyde (15 min, room temperature) followed by permeabilization with 0.1–0.2% Triton X-100 or saponin (5–10 min). For surface membrane targets, fixation without permeabilization may be sufficient. Block with 5–10% normal serum (matching the secondary antibody host species) for 30–60 min. Incubate primary antibody at the recommended dilution (IF 1:20-100) at 4°C overnight or 1–2 h at room temperature. Confirm nuclear/cytoplasmic localization of the target against published protein databases before designing your protocol.
This antibody has been experimentally validated in: Human. Cross-reactivity with other species has not been systematically evaluated. If your sample species is not listed, assess immunogen sequence homology to your target species ortholog. The immunogen used is: "Human IgG" — higher sequence identity between species increases the probability of cross-reactivity, but experimental validation with appropriate controls is necessary before drawing scientific conclusions.
Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. Storage Buffer: PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4. To maintain antibody activity, avoid repeated freeze-thaw cycles. Aliquot into single-use volumes before first freeze. If working with the antibody frequently, keep a working stock at 4°C for up to 4 weeks — add 0.02% sodium azide to the working stock as a bacteriostatic agent if azide is not already present in the buffer. Monitor performance against a validated positive control when transitioning between storage aliquots or lots.
Polyclonal antibodies are produced from immunized animals and may exhibit some degree of lot-to-lot variation in titer, background, and sensitivity. Each production lot is tested for performance against established acceptance criteria. When transitioning to a new lot, run a side-by-side pilot comparison against your previous lot using a validated positive control sample before updating your standard protocol. If significant performance differences are observed, contact our technical support team — titer adjustments may resolve the issue in most cases.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets — use code SIRNA10
20% Off Transmembrane Proteins — use code TM20
$50 Off All ELISA Kits — auto-applied at checkout (code ELISA50)
50% Off Lab Consumables + Free Shipping on orders $500+
15% Off Proteins from Trusted Suppliers — use code PROTEIN15
$99 Pipette Filler Promotion Package (reg. $236)
Save 10% on your first order of any R&D grade DENARASE® with code DENARASE10 at checkout.
