| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Glypican 2/GPC2 recombinant protein (Position: E25-D498) was used as the immunogen for the GPC2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GPC2 Antibody / Glypican 2 is a anti-GPC2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GPC2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Glypican-2 is predominantly expressed in the developing nervous system, where it regulates neurite outgrowth and synaptic organization. By binding heparan sulfate chains to morphogens and growth factors, GPC2 fine-tunes signal transduction gradients essential for brain patterning. The GPC2 antibody supports detection of this proteoglycan in neuronal cultures and brain tissue, allowing exploration of its role in axonal guidance and synaptic plasticity. GPC2 also interacts with cell adhesion molecules to influence neuronal connectivity during development.
Recent research has identified Glypican-2 as a cell surface oncoprotein in pediatric neuroblastoma. Overexpression of GPC2 enhances cell proliferation and tumor growth through sustained activation of oncogenic signaling pathways. The GPC2 antibody supports investigation of this function, enabling measurement of expression levels in tumor samples and evaluation of therapeutic targeting potential. Antibody-drug conjugates and immunotherapies directed against GPC2 are currently under study for selective tumor eradication.
Beyond neuroblastoma, Glypican-2 contributes to neuronal regeneration and neural stem cell regulation. Its heparan sulfate chains bind multiple signaling ligands, positioning GPC2 as a key modulator of microenvironmental signaling. The GPC2 antibody helps map these ligand�receptor interactions and track expression changes during neuronal differentiation. Reduced expression of GPC2 has been linked to impaired synaptic signaling, suggesting its importance in maintaining neural circuit stability.
The GPC2 antibody is validated for western blotting, flow cytometry, and immunofluorescence, showing clear cell surface localization consistent with GPI-anchored proteins.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
