| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse GPNMB recombinant protein (Position: R164-D564) was used as the immunogen for the GPNMB antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GPNMB Antibody / Transmembrane glycoprotein NMB / HGFN is a anti-GPNMB Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: GPNMB
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Functionally, GPNMB antibody identifies a 560-amino-acid glycoprotein with an extracellular domain containing an RGD-like integrin-binding motif, a transmembrane helix, and a short cytoplasmic tail with a tyrosine-based sorting signal. GPNMB facilitates cell-matrix interaction and promotes osteoblast differentiation, pigment cell function, and immune modulation. It is also upregulated during tissue repair and inflammation, reflecting its role in regeneration and immune regulation.
The GPNMB gene is located on chromosome 7p15.2 and encodes a protein expressed in bone, skin, brain, and immune cells. In macrophages and dendritic cells, GPNMB modulates cytokine release and antigen presentation, while in osteoclasts it contributes to bone resorption and remodeling. The protein undergoes ectodomain shedding, releasing soluble fragments that influence neighboring cells.
Pathologically, GPNMB is overexpressed in several cancers, including melanoma, breast, and glioma, where it promotes migration, invasion, and angiogenesis. It also plays roles in neurodegenerative diseases and inflammation. Because of its surface localization, GPNMB serves as a potential therapeutic target and biomarker. Research using GPNMB antibody supports studies of tumor biology, osteogenesis, and immune signaling.
GPNMB antibody is suitable for western blotting, immunohistochemistry, and flow cytometry to detect membrane-associated and soluble GPNMB.
Structurally, Transmembrane glycoprotein NMB features multiple N-glycosylation sites contributing to stability and cell-surface expression. The cytoplasmic domain interacts with intracellular adaptors, linking extracellular signals to intracellular pathways. This antibody enables detection of GPNMB in studies of cell communication, repair, and disease progression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
