| Field | Specification |
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| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human GPR35 was used as the immunogen for the GPR35 antibody. |
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Overview
GPR35 Antibody / G protein-coupled receptor 35 is a anti-GPR35 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GPR35
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
GPR35 antibody identifies a receptor that participates in multiple physiological and pathophysiological processes, including immune regulation, nociception, and gastrointestinal motility. It is expressed in leukocytes, monocytes, intestinal epithelial cells, and cardiomyocytes. The receptor also plays a role in chemotaxis and inflammatory signaling by regulating cytokine release and leukocyte migration. Ligand binding to GPR35 activates downstream pathways such as ERK1/2, RhoA, and PI3K-AKT, integrating metabolic and immune responses.
Structurally, GPR35 contains conserved transmembrane helices typical of GPCRs, an intracellular C-terminal tail that mediates beta-arrestin binding, and an extracellular N-terminal glycosylated region important for ligand recognition. The receptor's pharmacology has been extensively studied, and various endogenous and synthetic agonists-including kynurenic acid, lysophosphatidic acid, and zaprinast-have been identified. These ligands link GPR35 signaling to tryptophan metabolism and vascular regulation.
Clinically, GPR35 is associated with inflammatory and metabolic disorders. Genetic variants in the GPR35 gene are linked to inflammatory bowel disease (IBD), ulcerative colitis, and primary sclerosing cholangitis. In the cardiovascular system, GPR35 activation induces vasodilation and cardioprotective signaling, suggesting therapeutic potential for hypertension and ischemic injury. Conversely, aberrant activation has been implicated in cancer progression, particularly in gastric and colorectal carcinoma, where GPR35 may promote cell proliferation and migration.
Pathway analysis indicates that GPR35 functions within GPCR signaling networks, regulating MAPK and calcium-dependent pathways that affect cytokine expression and metabolic reprogramming. In the nervous system, it may influence pain sensitivity through modulation of sensory neuron excitability. The receptor's dual roles in immune and metabolic regulation position it as a promising drug target for inflammation and cardiovascular disease.
Immunohistochemical analysis using GPR35 antibody reveals membrane and cytoplasmic staining in immune and epithelial tissues, consistent with GPCR localization. The GPR35 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
