| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human RAI3/GPRC5A was used as the immunogen for the GPRC5A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GPRC5A Antibody / G-protein coupled receptor family C group 5 member A is a anti-GPRC5A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: GPRC5A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB
Biological background
Functionally, GPRC5A antibody identifies a 358-amino-acid transmembrane receptor localized to the plasma membrane and endosomal compartments. GPRC5A expression is strongly induced by retinoic acid and contributes to maintaining epithelial integrity and differentiation. It modulates multiple signaling pathways including EGFR, STAT3, and NF-kappaB, thereby influencing cell growth, polarity, and immune responses. Through its regulatory functions, GPRC5A acts as a context-dependent tumor suppressor in lung, pancreatic, and colorectal tissues.
The GPRC5A gene is located on chromosome 12p13.1 and is highly expressed in lung epithelium, gastrointestinal tract, and pancreas. In normal tissues, GPRC5A helps maintain epithelial homeostasis by dampening inflammatory and growth-promoting signals. Loss of GPRC5A expression has been implicated in lung adenocarcinoma and other epithelial cancers, where its absence leads to hyperactivation of EGFR and STAT3 pathways. Conversely, aberrant overexpression in certain cancers may promote proliferation and resistance to apoptosis.
In cell signaling, GPRC5A interacts with adaptor proteins to modulate receptor internalization and trafficking. It influences the balance between pro-inflammatory and anti-inflammatory signaling, acting as a mediator of cytokine responses in epithelial cells. GPRC5A also participates in differentiation processes during lung development and regeneration following injury, making it a valuable marker for epithelial cell identity and repair mechanisms.
GPRC5A antibody is used extensively in cancer biology, signal transduction, and epithelial cell research. It is applicable for immunoblotting, immunohistochemistry, and flow cytometry to evaluate receptor expression patterns and localization. In oncology, GPRC5A serves as a biomarker for tumor progression and epithelial transformation. Its detection aids in understanding the cross-talk between retinoic acid signaling and oncogenic pathways.
Structurally, GPRC5A possesses seven transmembrane helices typical of GPCRs, with an extracellular N-terminus and intracellular C-terminal domain involved in signaling interactions. It lacks a known G-protein coupling profile, suggesting atypical regulatory mechanisms through scaffolding proteins.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
