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| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human GSTM2 was used as the immunogen for the GSTM2 antibody. |
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Overview
GSTM2 Antibody / Glutathione S-transferase Mu 2 is a anti-GSTM2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GSTM2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB
Biological background
Functionally, GSTM2 antibody identifies a 218-amino-acid enzyme belonging to the Mu class of the glutathione S-transferase family. GSTM2 participates in phase II detoxification pathways by binding and neutralizing toxic metabolites, drugs, and environmental carcinogens. It exhibits substrate specificity toward lipid peroxidation products, reactive oxygen species intermediates, and xenobiotic compounds. GSTM2 is also involved in modulating signaling pathways such as JNK and MAPK, linking oxidative stress defense to cell survival and apoptosis regulation.
The GSTM2 gene is located on chromosome 1p13.3 and is primarily expressed in liver, skeletal muscle, and brain. Its expression levels vary between individuals due to polymorphisms in the GSTM gene cluster, influencing susceptibility to toxins and disease. As a member of the Mu family, GSTM2 functions alongside other isoenzymes (GSTM1-GSTM5) to maintain redox balance and detoxification capacity.
Pathologically, reduced GSTM2 expression or polymorphic variants have been associated with increased risk of cancer, neurodegenerative disease, and drug-induced toxicity. Elevated GSTM2 levels can enhance cellular resistance to chemotherapy and oxidative damage. Research using GSTM2 antibody supports studies in toxicology, pharmacogenomics, and oxidative stress biology.
GSTM2 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect detoxification enzymes.
Structurally, Glutathione S-transferase Mu 2 forms a homodimer with each subunit containing a glutathione-binding (G-site) domain and a hydrophobic substrate-binding (H-site). Its tertiary structure supports efficient conjugation reactions and dynamic interaction with glutathione substrates. This antibody enables investigation of GSTM2's function in metabolic detoxification and cellular stress response.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
